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Coding

Part:BBa_K2984065:Design

Designed by: Darius Rauch   Group: iGEM19_Humboldt_Berlin   (2019-10-16)


Free Slot


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1106
    Illegal PstI site found at 1453
    Illegal PstI site found at 1826
    Illegal PstI site found at 2521
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1106
    Illegal PstI site found at 1453
    Illegal PstI site found at 1826
    Illegal PstI site found at 2521
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 4
    Illegal BamHI site found at 1085
    Illegal XhoI site found at 1271
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1106
    Illegal PstI site found at 1453
    Illegal PstI site found at 1826
    Illegal PstI site found at 2521
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1106
    Illegal PstI site found at 1453
    Illegal PstI site found at 1826
    Illegal PstI site found at 2521
    Illegal NgoMIV site found at 1208
    Illegal NgoMIV site found at 2095
    Illegal NgoMIV site found at 2998
    Illegal AgeI site found at 2036
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Secretion construct for C.reinhardtii designed for transformation in Clamydomnasd and further cloning into Level 2 backbone with MoClo standard.



Source

The construct consist of the five different Level 0 parts PsadIntron (BBa_K2984046), bleRscp (BBa_K2984045), ARS (BBa_K2984000), YFP (BBa_K2984017) and Rbcs2(BBa_K2984018) which were cloned into the level 1 Backbone (BBa_K2984002) using the MoClo standard


References